show Abstracthide AbstractTef [Eragrostis tef (Zucc.) Trotter, also known as teff] is an allotetraploid (2n = 4x = 40) C4 cereal crop, originating in Ethiopia. Tef is characterized by high grain and feed nutritional qualities, and resilience to abiotic and biotic stresses, thus it holds a great potential to sustain food and nutrition security in Africa and other parts of the world. The objective of our study was to identify genomic regions associated with major traits of interest. Tef accessions, mostly collected by the USDA in Ethiopia during the 1950s and 1960s and later stored in the Israel Plant Gene Bank, were propagated, genetically fixed via one round of single-plant selection, and subjected to GBS. A tef diversity panel (TDP-300) consisting of 297 genotypes was assembled. The data generated can facilitate studies on the genetic basis underlying major traits in tef, as well as marker-assisted breeding.